Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVQWGIELLKSAGLFFLHPLFWFFIIITLAFGYVRIKRERKTFHTRIADIYDDLKFTYTKGLIPGLLLSVILGGLGISIPLGLLAIIAVITAAAAFTLRANWMSAAYIVSVSMLIGFGLQIYQAEPFLERFPQGFAVVWPAVAVFLGLLIITEGAVAYRSAHVRTSPALVVSSRGLPIGQQLANRVWLLPLFLLVPGNGLESHLSWWPVFTVPGGSFHFLWIPYFVGFGQRVQGSLPETSIRITAKRVCILGLAVAVLGAASLLWTPLAGAAVCTALLGRIFLSIKQRVNDNAAPFYFSKRDQGLMVLGIIPNTPAEDLELKIGEIITKVNGIPVKNVSDFYEALQHNRAYVKLEIIGLNGEIRFDQRASYEGEHHELGILFVKDDREDEAVASGS 4RI0 Chain:A ((253-322)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VSSGIYVQEVAPNSPSQRGGIQDGDIIVKVNGRPLVDSSELQEAVLT-ESPLLLEVRRGNDDLLFSIAPEV-------------------------

General information: TITO was launched using: RESULT: Template: 4RI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 270 -25511 -94.48 -364.44 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.44 3D Compatibility (PKB) : -94.48 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.617

(partial model without unconserved sides chains): PDB file : Tito_4RI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RI0-query.scw PDB file : Tito_Scwrl_4RI0.pdb: