Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYNIRGENIEVTPALKDHVERKIGKLERYFDHSVDADVNVNLKFYNDKESKVEVTIPMTDLALRSEVHNEDMYNAIDLATNKLERQIRKHKTKVNRKFREQGSPKYLLANGLGSDTDIAVQDDIEEEESLDIVRQKRFNLKPMDSEEAILQMNMLGHNFFVFTNAETNLTNVVYRRNDGKYGLIEPTE 1L4S Chain:A ((1-112)) -TMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE---PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGE------------ARRAATSVKDANFVEEVEEE-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1L4S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 351 -39240 -111.79 -350.35 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -111.79 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_1L4S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L4S-query.scw PDB file : Tito_Scwrl_1L4S.pdb: