Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGELANYKRLEKVGEGTYGVVYKALDLRPGQGQRVVALKKIRLESEDEGVPSTAIREISLLKELKDDNIVRLYDIVHSDAHKLYLVFEFLDLDLKRYME-----GIPKDQPLGADIVKKFMMQLCKGIAYCHSHRILHRDLKPQNLLINKDGNLKLGDFGLARAFGVPLRAYTHEIVTLWYRAPEVLLGGKQYSTGVDTWSIGCIFAEMCNRKPIFSGDSEIDQIFKIFRVLGTPNEAIWPDIVYLPDFKPSFPQWRRKDLSQVVPSLDPRGIDLLDKLLAYDPINRISARRAAIHPYFQES 4EOJ Chain:A ((3-292)) --GSMENFQKVEKIGEGTYGVVYKARNKLTGE---VVALKKIRL----EGVPSTAIREISLLKELNHPNIVKLLDVIHTE-NKLYLVFEFLSMDLKDFMDASALTGIP------LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQD-

General information: TITO was launched using: RESULT: Template: 4EOJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -180191 for 2216 contacts (-81.3/contact) + 2D Compatibility (PS) -29559 + (NN) -9245 + (LL) 420 1D Compatibility (HY) -38800 + (ID) 9250 Total energy: -266625.0 ( -120.32 by residue) QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_4EOJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EOJ-query.scw PDB file : Tito_Scwrl_4EOJ.pdb: