Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASPSNDSTAPVSEFLLICFPNFQSWQHWLSLPLSLLFLLAMGANTTLLITIQLEASLHQPLYYLLSLLSLLDIVLCLTVIPKVLAIFWFDLRSISFPACFL-QMFIMNSFLTMESC--TFMVMAYDRYVAICHPLRYPSIITDQFVARAVVFVIARNAFVSLPVPMLSARLRYCAGNIIKNCICSNLSVSKLSCDDITFNQLYQFVAGWTLLGSDLILIVISYSFILKVVLRIKAEGAVA-------------------KALSTCG-SHFILILFFSTVLLVLVITNLARKRIPPDVPILLNILHHLIPPALNPIVYGVRTKEIKQGIQNLLKRL 2YCW Chain:A ((8-297)) -----------------------QQWEAGMSLLMALVVLLIVAGNVLVIAAIGSTQRLQTLTNLFITSLACADLVVGLLVVPFGATLV---VRGTWLWGSFLCELWTSLDVLCVTASIETLCVIAIDRYLAITSPFRYQSLMTRARAKVIICTVWAISALVSF-LPIMMHWWRDEDPQALK-------CYQDPGCCDFVTNRAYA-------IASSIISFYIPLLIMIFVALRVYREAKEQIRKIDRASKRKRVMLMREHKALKTLGIIMGVFTLCWLPFFLVNIVNVFNRDLVPDWLFVAFNWLGY-ANSAMNPIIY-CRSPDFRKAFKRLLAF-

General information: TITO was launched using: RESULT: Template: 2YCW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -246290 for 2006 contacts (-122.8/contact) + 2D Compatibility (PS) -26987 + (NN) -9119 + (LL) 2208 1D Compatibility (HY) -29200 + (ID) 3600 Total energy: -312988.0 ( -156.03 by residue) QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_2YCW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YCW-query.scw PDB file : Tito_Scwrl_2YCW.pdb: