Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLSPAIFSIGPVSIHWYSL-AYVLGIVFAYWHLHKLDEQKIFTKNFYDSLLTATIIGII-----------LGGRLGFVLIYDPISYINNPIEILKTWKGGMSFHG----GAIGVLCAVIISCRRYNIPI-------FYTLDLISC----------GVPIGLF-LGRIGNFVNGELFGRV--TTMPWGMVFPESGDNLLRHPSQLYEAFFEGLLFFAIANSLFFLTR--IRLYHGTLTGIAVIWYGTVRFVVE--FFREPDYQVG---YLWLDLTMGQLLSIFMT-------LLGIIVYLSALNSKFNTKSVA------------------- 4NTC Chain:A ((2-326)) MSIG-KLLSNGALLVDVLIIGAGPAGLSTATGLARQLHTAVVFDSGVYRNAKTQHMHNVLGWDHRNPAELRAAGRADLTTRYSTIQFQNSTIEAIRQVETNQLFEARDNEGHSWYGRKVVLATGVRDIPLDIEGYSECWANGIYHCLFCDGYEERGQETVGVLALGPIANPARALHLARMALRLSESVTIYTNGNEQLAKEIQQAAEESPVG------ASGLKFEARPIRRFEKGDVAKTVIVHLGESESKTEGFLVYNPQTEVNGPFAKQLALNMTEGGDILTTPPFYETSVPGVFAVGDCATPLKAVTPAVSMGSLAAGGLVAQLQAQAL

General information: TITO was launched using: RESULT: Template: 4NTC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -226212 for 1891 contacts (-119.6/contact) + 2D Compatibility (PS) -27425 + (NN) -1406 + (LL) 824 1D Compatibility (HY) -2000 + (ID) 2500 Total energy: -258719.0 ( -136.82 by residue) QMean score : 0.155

(partial model without unconserved sides chains): PDB file : Tito_4NTC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NTC-query.scw PDB file : Tito_Scwrl_4NTC.pdb: