Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTIRTTQMQVDHKTSLIIDAVEKFGGEVRLVGGCVRDSILQRKVHDIDLATNLLPSQVIEALKFHNIKAIPTGLKHGTITAVLNQRSFEITTLRHDVKCDGRHAKVEFTDNWRADASRRDFTFNALYANKYGHIYDYFGGTQDLKARRLNFIGNAEDRIKEDYLRILRAFRFHAKIC--IGDMSDKILNVCKKHAHKIYNLSGERIRDEMFKLLEYDAPVPTLKSMQKSDVLQKI-IPKEVKCE----ILSSAFLFGTDALVKLALLLRTTEKNDRLSLGEYVSKFLRLSNKQKKKLLFLLSNN---IKTELTEKEQRKYISLLGKELYCD-LVKVCGVESGANVDEYISLAQVLNIQKFPLSGDDLINIGYQPGKNLGRNLKLLRQHWEDSSYTLTKKELMLYAKSLL 4X4W Chain:B ((32-409)) ---------------------------ELRIAGGAVRDLLNGVKPQDIDFATTATPTQMKEMFQSAGIRMIN-----GTITARLHEENFEITTLRIDVTTD---AEVEFTTDWQKDAERRDLTINSMFLGFDGTLFDYFNGYEDLKNKKVRFVGHAKQRIQEDYLRILRYFRFYGRIVDKPGDHDPETLEAIAENAKGLAGISGERIWVELKKILVGNHVNHLIHLIYDLDVAPYIGLPANASLEEFDKVSKNVDGFSPKPVTLLASLFKVQDDVTKLDLR------LKIAKEEKNLGLFIVKNRKDLIKATDSSDPLKPYQDFIIDS---DATTRVCELLKYQGEHCLLKEMQQWSIPPFPVSGHDIRKVGISSGKEIGALLQQLREQWKKSGYQMEKDELLSYIKKTL

General information: TITO was launched using: RESULT: Template: 4X4W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -146353 for 2663 contacts (-55.0/contact) + 2D Compatibility (PS) -37593 + (NN) -11228 + (LL) 3152 1D Compatibility (HY) -22400 + (ID) 6450 Total energy: -220872.0 ( -82.94 by residue) QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_4X4W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X4W-query.scw PDB file : Tito_Scwrl_4X4W.pdb: