Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFSNLYRGLLKTSSRFSDGVKSIFSAKKKLDQSLLDELEELLISMDIGHKTSKLIIDRLASIKFDKEVE-HNIITQQLMSEIEAILNPVVQPLILDK-NPHIIMVCGVNGNGKTTTIGKLAYKYKNMGKSVMLVACDTFRAAASEQLNIWAERSDCFIVTGEYGNDSASVAYRAVSQAIKGSVDVVLIDTAGRLQNNVNLMEELSKIYRTIKKLDGTAPHDVILVLDATTGQNAYSQLEAFSKMVSVTGLIITKLDGTAKGGVVIGLAETYRVKLHAIGIGESIEDLREFTSKEFAKALFGCD 2YHS Chain:A ((195-495)) FFARLKRSLLKTKENLGSGFISLFRG-KKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFAR-

General information: TITO was launched using: RESULT: Template: 2YHS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -250446 for 2594 contacts (-96.5/contact) + 2D Compatibility (PS) -32570 + (NN) -15310 + (LL) -12 1D Compatibility (HY) -25600 + (ID) 7100 Total energy: -331038.0 ( -127.62 by residue) QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2YHS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YHS-query.scw PDB file : Tito_Scwrl_2YHS.pdb: