Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIPQLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQHAMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTVSISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVYDNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKEEATRRLLNNGDIVKRAIRDRQP 4S12 Chain:C ((11-298)) ------SESRNPDTMDLDTLSTLEMLTRINDEDRKVPEAIRLVIPNIAQAVDLAAKALRDGGRLIYLGAGTSGRLGVLDASECPPTFGVPHGRVIGLIAGGPGALLKAVEGAEDDVSLGERDLRDLQLTATDMVVGLAASGRTPYVIGALRFARQLGCPTAAISCNPDSPIAQEALVAISPVVGPEALTGSTRMKSGTAQKLVLNMLSTGAMVKLGKVYQNLMVDVKATNVKLVDRACRIVVEATGASRVEAENALSQTEFEVKPAILMILKGVSVEQARLNLQQHNGYLRAAL-----

General information: TITO was launched using: RESULT: Template: 4S12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -173407 for 2446 contacts (-70.9/contact) + 2D Compatibility (PS) -30228 + (NN) -7537 + (LL) 568 1D Compatibility (HY) -21600 + (ID) 7200 Total energy: -239404.0 ( -97.88 by residue) QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_4S12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4S12-query.scw PDB file : Tito_Scwrl_4S12.pdb: