Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGVYIISLKESQRRLDTEKLVLESNEKFKGRCVFQIFDAISPKHQDFEKLLQELYNAQSLLQSDWYHSYVGAGLTLPELGCYLSHYLLWKECVKLDQPVVILEDDVTLESHFMQALEDCLKSPFDFVRLYGCYWYYH---ETKFHVLPKEFVFPPFDHSFKNNPILEKFKKFFDVSRFLNLSTHKVIHYILKKIQKSYYATHEKEAFFLEHFYLTSVYVASTAGYYLTPKGAKTFIEATESFKIIEPVDMFMDNSAYHDVANLTYVPCPVSLSEHSLDSTIQKPQKKSLKSYPLPPQKSTFKNLFYYSLNAKKRLNAFQQYSKQYAPLKTPKEV 3SPA Chain:A ((1034-1125)) --------------------------------------------------------------------------------------------------------------SHMMLTALHCYRKGLTFVSVHDCYWTHAADVSVMNQVCREQFV------RLHSEPILQDLSRFL-VKRFCS-EPQKILE--ASQLKETLQAVPKPGAFDLEQ---------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3SPA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -22853 for 479 contacts (-47.7/contact) + 2D Compatibility (PS) -9514 + (NN) -1961 + (LL) 17040 1D Compatibility (HY) -8800 + (ID) 1250 Total energy: -27338.0 ( -57.07 by residue) QMean score : 0.089

(partial model without unconserved sides chains): PDB file : Tito_3SPA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SPA-query.scw PDB file : Tito_Scwrl_3SPA.pdb: