Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSWMKKKYFTLLLQSSVVLAVFIGCSSTRNHTFSALSNQENTDDKLPVVHSIKTINDVSSVGFEWSKVADTYDIDGFVLYRLKKD-SKLKRIATIKNPYATHYYDEGLETESSYTYQLATYKGDKISKLSEPILVKTSFINPVESVFASLEYPKSVKVFWSPHPNPSVSKYIIQRQN-KD-GKFLNVGAVKNRLFVEFFDKDLEDGQKYRYQIIAENFMGDKSRPSVIVEGKTKDLPKEIANVRVSQNLTRQIELSWDKSPEEDVIAYRIYASNNRNDK-Y-KFIAQTTNTSYVDKIEKDNLTRYYKVVAVDKTHLEGALPKEPAMGETSDRPEAPIITKGTI-QDSSALIQWENNPSPKIATYAVYRFEANSKT--PLRFGNITQNQFVDKDMKVGVAYRYQVVSVDKDGLESHPSKEVRLFLER 1FNF Chain:A ((1145-1500)) ----------------------------------------------PPTNLHLEANPDTGVLTVSWERSTTP-DITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTI-I-PAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAEL-SI-SPSDNAVVLTNLLPGTEYVVSVSSVYEQH-ESTPLR---GRQKTGLDSPTGIDFSDITANSFTVHWIAPR-ATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREE-SPL-LI---GQQSTVSDVPRDLEVVAATPTSLLISWDAPA-VTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGD-SPASS--------

General information: TITO was launched using: RESULT: Template: 1FNF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -6841 for 2515 contacts (-2.7/contact) + 2D Compatibility (PS) -37439 + (NN) -12999 + (LL) 5448 1D Compatibility (HY) -3600 + (ID) 2700 Total energy: -58131.0 ( -23.11 by residue) QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_1FNF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FNF-query.scw PDB file : Tito_Scwrl_1FNF.pdb: