Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKQIISWITDYQNTGDEAVLRQVREVCWPIVEAVLQEKVMDDEQANNLREKGIERFPFIISKYQADVQLPVETFLQNTYRFYFHQVMRESS 5IPL Chain:F ((76-154)) ----EVYFARRAL-RGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTI--------

General information: TITO was launched using: RESULT: Template: 5IPL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 276 -44825 -162.41 -567.40 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain F : 0.63 3D Compatibility (PKB) : -162.41 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_5IPL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IPL-query.scw PDB file : Tito_Scwrl_5IPL.pdb: