Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNLNEKQLITEIVGLARSQGLTVHSENAQLNETGMDFQVVFAKDDTGMPWVLRKPRRSDVVERASAEGITLAFLRAN--LTADVPDWRIHT----PELIAYPMLKGTPAAGIDLEQKQYVWNMDHQPPSDDFVRTLADILAELHGTDQISAGQSGIEVIRPEDFRQMTADSMVDVKNKL-GVSTTLWERWQKWVDDDAYWPGFSSLIHGDLHPPHILIDQNGRVTGLLDWTEAKVADPAKDFVLYQTI----FGEKETARLLEYYDQAGGRIWAKMQEHISEMQAAYPVEIAKLALQTQQEEHINMALEALGVTSD 4ORK Chain:A ((9-299)) ------ATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVN----NEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEI-------YSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID--NKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDI---EKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIY----

General information: TITO was launched using: RESULT: Template: 4ORK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1294 -112984 -87.31 -403.51 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -87.31 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_4ORK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ORK-query.scw PDB file : Tito_Scwrl_4ORK.pdb: