Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKVGLVDDYRVDLEKLEAIVSRM-Q--DVEI-VFSTDSAKEAYRRVK-NGDIDLLLADIEM--PHMSGYELADLIKSHSLDVDVIFVTGHGGY--AVHAFDLNVHDYIMKPYYADRLAASFDRYLKKKTE-----TSLNGRILIKQKSEMHVLQKKDIIFAERTGR--STTIVTTAEEVQTYQTLNDIKGDLPEKD-FLRSHRSFIINIHYIKHFSAYTKHSFTVSFEGTSKKAMITKQQLDYFQNYYF 4CBV Chain:A ((2-244)) --KVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEEYIENDEVNQLYFLAIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQVSALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILYIETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRKN-L--EIVFYEDH-RCPISRLKIRKLKDIL-

General information: TITO was launched using: RESULT: Template: 4CBV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1042 -133743 -128.35 -591.78 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -128.35 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_4CBV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CBV-query.scw PDB file : Tito_Scwrl_4CBV.pdb: