Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNKQLKKKAQEIGNVPTH--YELEIEDYDQKQKKYGQAYFIWKDPKDPEKHITVELRN-DGALLTFSTTVHSETDKKLPDAELKLTALQFAAANHPGTFMNFHFQGKEERGQHIRFVYTKM-ELGLPIPNSGFLIDMTR-SGQIVHFLYYGE---GHKAEVPTEFVAKEKVVSHYLNTMSFE---LMYDVIDGEQEPRLVYEPILP------GYSYPA-DVDEIVPDQHIADERIENTAPLPPLQNKEEVDIFALLGFTSDMQKVSERDFGEQIGSTWRRGAAPERKDLSIGSYFETRNKNTIKMKTDKRTGKLKAALSFMDWRNNLQCSTEECQEIALQFLYALYPRAAEFFRVNPVRIDERGRVRNHFSVWYKGVPLRFGAARIIVNPETGLIDAFMAPDIEPEQLEAINHRPDVSAEEAKEAFLAAFDVKLEWQPDFTAGSDQHCKLVYKPVYPSYIDAHIRKKKRL 5BOI Chain:A ((11-229)) -SEDVAKQVARSFLNLKGNEQIHIVKSG-KD--ADYEVYSLTITDPK-TNQETYMDITQKGGYPLWVLEDRDI-KKQNISLNDAMNKATKFLKDHRFE---SLVMAESAQYDNMGVFTFVEQTESGVRIYPDSVKMKMSLEDGSVIGFSAKDFLLKHRTRDIPKPKISKEQAKTKLNSNVKVMEERLAIITND-LNEEVLCYEFLGTIKNDTYRIFINADTGFEEKVEK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5BOI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 894 -91303 -102.13 -454.24 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -102.13 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_5BOI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BOI-query.scw PDB file : Tito_Scwrl_5BOI.pdb: