Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWEQLYDPFGNEYVSALVALTPILFFLLALTVLKMKGILAAFLTLAVSFFVSVWAFHMPVEKAISSVLLGIGSGLWPIGYIVLMAVWLYKIAVKTGKFTIIRSSIAGISPDQRLQLLLI-GFCFNAFLEGAAGFGVPIAISAALLVELGFKPLKAAALCLIANAASGAFGAIGIP---VITGAQI--GDL-SALELSRTLMWTLPMISFLIPFLLVFLLDRMKGIKQTWPALLVVSGGYTAVQTLTMAVLGPELANILAALFSMGGLALFLRK-WQPKEIYREEGAGDAGEKKAYRAADIAKA---WSPFYILTAAITIWSLPAFKALFQEGGLLYQSTLLFKMPFLHQQIMKMPPIAPSAMPLDAVFKVDLLSATGTAILAAVIVTGLF---SKKF-S----SRDAFASLKETGKELWVPIMTICFVMGFANLANFAGLSSS-IGLALAKT--GDL----FPFVSPVLGWIGVFITGSVVSNNALFGHLQVVTGAQIGAGSDLLLAANTAGGVMAKLVSPQSIAI--AAAAVGQTGK---ESKLFKRTVAYSLILLLIICIWTFILARLGV 4R0C Chain:A ((66-486)) -----------------------------------------------------------LRYLFTTLVGNFTG-FAPLG-VVLVAMLGLGVAEQSGLLSVSLASLVRRSSGGALVFTVAFAGVLSSLT-VDAGYVVLIPLAGLVFQLAGRPPIAGIATAFAAVSGGFSANLLVGPVDATLAGLSTEAAHIIDPD-R-------------TVAAT-----G---------------NYWFIIASTFLV------------T-GL---VTLITRTLTEPRLAHANTVADASVDAPQIHSRAMKWTGLTLAILLAGLALLVL----PNDAPLRHP--------------------------D----TGSVLGSP---FIHGLVVIVALIAGICGAVYGRVSGQFRNSGAVITAMEVTMASMAGYLVLMFFAAQFVAWFNYSQLGLLLAVKGAAWLGALTVPKVVLLLLFVVLTALINLMIGSASAKWSILAPVFIPMLMLLGISPEASQAAYRVGDSSTNIITPLMPYFVLVLGFARRYQPETGIGTLIALMLPYSLTLLLGWSVLLGVWIGF--

General information: TITO was launched using: RESULT: Template: 4R0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1891 -281611 -148.92 -722.08 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -148.92 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_4R0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R0C-query.scw PDB file : Tito_Scwrl_4R0C.pdb: