Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTRNSRSELEKTAVQLFRKLGTRTVLFHQAAAQALGLFPTDLKSADI----------LNEAGPMTAGELGKKTGLSTGSVTALVDRLEKAGYVAREKDPNDRRRVVIVPLTASKKHIKDLFRPLSESTMDLCREYTEEELELIFSFVGKAADIMEEELERLKQ 4GXO Chain:A ((13-129)) --------------------------LFYVSSKEIIKKYTNYLKEYDLTYTGYIVLMAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQI-SLTEQGKAIKS---PLAEISVKVFNEFNISERE--------ASDII---------

General information: TITO was launched using: RESULT: Template: 4GXO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 245 -24023 -98.05 -224.51 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -98.05 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_4GXO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GXO-query.scw PDB file : Tito_Scwrl_4GXO.pdb: