Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAKRAIPVRERNIVLIGFMGVGKTTIGQLVAKKLYRDFIDIDQQIEKDFNMSIPEIFEKKGEDFFRKTEKEYILDICHHKRFKIVSLGGGSFKQEEIRNCCLENCLVLHLDLSWENWKQRADLLIE-SRPV-LHNRSMDEMEQLFNERKVIYDKHNSKV-ATDNLSPEEVADYIVETLKIGWDLYQPM 3TRF Chain:A ((7-173)) ------------NIYLIGLMGAGKTSVGSQLAKLTKRILYDSDKEIEKRTGADIAWIFEMEGEAGFRRREREMIEALCKLDNI-ILATGGGVVLDEKNRQQISETGVVIYLTASIDTQLKRIGQKGEMRRPLFIKNNSKEKLQQLNEIRKPLYQAMADLVYPTDDLNPRQLATQILVDIK---------

General information: TITO was launched using: RESULT: Template: 3TRF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 715 -80546 -112.65 -491.13 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -112.65 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_3TRF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TRF-query.scw PDB file : Tito_Scwrl_3TRF.pdb: