Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLLLALLPALFWGSIVLFNVKLGGGPYSQTLGTTIGALIVSIVIYFFVQPVLSLRIFIVGIVSGLFWSLGQANQLKSIQLMGVSKTMPISTGMQLVSTSLFGVIVFREWSTPIAITLGVLALIFIIVGIILTSLEDKNDKKEGEPSNLKKGILILLVSTLGYLVYVVVARLFNVSGWSALLPQAIGMVVGGLVLTYRHKPFNKYAIRNILPGLIWAGGNMFLFISQPRVGVATSFSLSQMGIVISTLGGIFILREKKTKRQLIAIAIGIILIIAAAVFLGIAKTNS 2JO1 Chain:A ((22-37)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VIAGILFILGILIVLS-----

General information: TITO was launched using: RESULT: Template: 2JO1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 5 -1618 -323.60 -101.13 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.38 3D Compatibility (PKB) : -323.60 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.38 QMean score : 1.170

(partial model without unconserved sides chains): PDB file : Tito_2JO1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JO1-query.scw PDB file : Tito_Scwrl_2JO1.pdb: