Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVFILFYLWIVPIVIGILCSVAAHKSKGKMRVAPGIAMIVLSIISLITAFTAGHTNFHVFIGG------MFLFGTFLVGSAFPFFFGLKKKEK 3S8F Chain:A ((243-318)) ------VYFWLLP-AYAII-YTILPKQAGGKL--VSDPMARLAFLLFLLLSTPV-GFHHQFADPGIDPTWKMIHSVLTLFVAVPSLM-------

General information: TITO was launched using: RESULT: Template: 3S8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 153 -31483 -205.77 -449.75 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -205.77 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.049

(partial model without unconserved sides chains): PDB file : Tito_3S8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3S8F-query.scw PDB file : Tito_Scwrl_3S8F.pdb: