Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAARWHNQKDIRIEHIEEPKTEPGKVKIKVKWCGICGSDLHEYLGGPIFIPVDKPHPLTNETAPVTMGHEFSGEVVEVGEGVENYKVGDRVVVEPIFATHGHQG----AYNLDEQMGFLGLAGGGGGFSEYVSVDEELLFKLPDELSYEQGALVEPSAVALYAVRSSKLKAGDKAAVFGCGPIGLLVIEALKAAGATDIYAVELSPERQQKAEELGAIIV-DPSKTDDVVAEIAERT--G-GGVDVAFEVTGVPVVLRQAIQSTTIAGETVIVSIWEKG--AEIHPNDIVIKERTVKGIIGYRDIFPAVLSLMKEGYFSADKLVTKKIVLDDLIEEGFGALIKEKSQVKILVRPN 4EJ6 Chain:A ((24-362)) MKAVRLESVGNISVRNVGIPEPGPDDLLVKVEACGICGTDRHLLHGEFP------------STPPVTLGHEFCGIVVEAGSAVRDIAPGARITGDPNISCGRCPQCQAGRVNLCRNLRAIGI-HRDGGFAEYVLVPRKQAFEIPLTLDPVHGAFCEPLACCLHGVDLSGIKAGSTVAILGGGVIGLLTVQLARLAGATTVILSTRQATKRRLAEEVGATATVDPSAGD-VVEAIAGPVGLVPGGVDVVIECAGVAETVKQSTRLAKAGGTVVILGVLPQGEKVEIEPFDILFRELRVLGSFINPFVHRRAADLVATGAIEIDRMISRRISLDEAP-DVISNPAAAG-EVKVLVIP-

General information: TITO was launched using: RESULT: Template: 4EJ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2043 -231329 -113.23 -703.13 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -113.23 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_4EJ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EJ6-query.scw PDB file : Tito_Scwrl_4EJ6.pdb: