Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILRREGISFDGMLARQVSEQDLDDFDYIIAMDAENIGSLRSMAGFKNTSHIKRLLDYVEDSDLADVPDPYYTGNFEEVCQLIKTGCEQLLASIQKEKQL 4ETM Chain:A ((18-173)) MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILRREGISFDGMLARQVSEQDLDDFDYIIAMDAENIGSLRSMAGFKNTSHIKRLLDYVEDSDLADVPDPYYTGNFEEVCQLIKTGCEQLLASIQKEKQL

General information: TITO was launched using: RESULT: Template: 4ETM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 813 -113161 -139.19 -725.39 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -139.19 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.684

(partial model without unconserved sides chains): PDB file : Tito_4ETM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ETM-query.scw PDB file : Tito_Scwrl_4ETM.pdb: