Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQNTKLRPITPEFDPWEAYMDVDQYGDMQLTNVEFTTTTLCNMRCEHC-----AVGYTLQPKDPNALPIDLLLKRLEEIPRLRSISITGGEPMLSLKSVKEYVVPLLKYAHERG--VRTQINSN-LTLDIERYEWIIPYLDVLHISHNWGTVEDFAEIGFAMMDRKPTFEQRARYFEKMIENSRTLVDAGVMVSAETMLNKRTLPHIEHIHRQITE--DMKCQRHEVHPMYPSDFASALESLSLKEMRQAIHRLLDIRDENTWMLFGTLPFYACSPDEDDQKLLRRLRAAKNVTVRNDPDGR------SRLNVNIFDGNIIVTDF--GDTPPLGNIQTDSLPSAYEKWRETKLAK--------------ELSCH--CPNVRCLGPNVLVK-NSYYQDTDFTKRQARS------------------------ 4K36 Chain:A ((2-392)) -------PPLSLLIKP---------------------ASSGCNLKCTYCFYHSLSDNVKSYGIMRDEVLESMVKRVLNEANGHCSFAFQGGEPTLAGLEFFEKLMELQRKHNYKNLKIYNSLQTNGTLIDESWAKFLSENKFLVGLSMD-GPKEIHNLN------RKDCCGLDT--FSKVERAAELFKKYKVEFNILCVVTSNTARHVNKVYKYFKEKDFKFLQFINCLDPLYEEKGKYNYSLKPKDYTKFLKNLFDFWYEDFL--------------NGNRVSIRYFDGLLETILLGKSSSCGMNGTCTCQFVVESDGSVYPCDFYVLDKWRLGNIQDMTMKELFETNKNHEFIKLSFKVHEECKKCKWFRLCKGGCRRCRDSKEDSALELNYYCQSYKEFFEYAFPRLINVANNIVDKLAAALEHHHHHH

General information: TITO was launched using: RESULT: Template: 4K36.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1579 -104178 -65.98 -323.53 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -65.98 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.247

(partial model without unconserved sides chains): PDB file : Tito_4K36.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K36-query.scw PDB file : Tito_Scwrl_4K36.pdb: