Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWKEKVSVTPPYHFDRVLDRLSLDPLNAVD-REAREVRVPIRNQAGDVCIVKVQALGHAGEPEFLVSGET----DQGEMMKEIKRIFQWENHLQHVLDHFSKTSLSAIFEEHAGTPLVLDYSVYNCMMKCIIHQQLNLSFAYTLTERFVHAFGEQK---DGVWCYPKPETIAELDYQDLRDLQFSMRKAEYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLMFGLGRPNLFPLADIGLQNAIKRHFQLDDKPAKDVMLAMSKEWEPYLSYASLYLWRSIE 1MPG Chain:A ((2-275)) ---YTLNWQPPYDWSWMLGFLAARAVSSVETVADSYYARSLAVGE-YRGVVTAIPDI--ARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIV-----NGALGRLGAARPGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQRLAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTI--PGDVEQAMKTLQTFPGIGRWTANYFALRGWQAKDVFLPDDYLIKQRFP---G----MTPAQIRRYAERWKPWRSYALLHIWYTE-

General information: TITO was launched using: RESULT: Template: 1MPG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1346 -161672 -120.11 -607.79 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -120.11 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_1MPG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MPG-query.scw PDB file : Tito_Scwrl_1MPG.pdb: