Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAHELKEALETLKETGVRITPQRHAILEYLVNSMAHPTADDIYKALEGKFPNMSVATVYNNLRVFRESGLVKELTYGDASSRFDFVTSDHYHAICENCGKIVDFHYPGLDEVEQLAAHVTGFKVSHHRLEIYGVCQECSKKENH 3F8N Chain:B ((4-139)) ---HELKEALETLKETGVRITPQRHAILEYLVNSMAHPTADDIYKALEGKFPNMSVATVYNNLRVFRESGLVKELTYGDASSRFDFVTSDHYHAICENCGKIVDFHYPGLDEVEQLAAHVTGFKVSHHRLEIYGVCQEC------

General information: TITO was launched using: RESULT: Template: 3F8N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 450 -70567 -156.82 -518.88 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.98 3D Compatibility (PKB) : -156.82 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_3F8N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8N-query.scw PDB file : Tito_Scwrl_3F8N.pdb: