Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKTKLTGRIVTRDDPDYNEARTNINLSLERYPDIIVFCQNKQDALNALKWARENRVPFRIRGGRHSYENFSLLNNGLVIDLSEMKKITVNQDKKLAYIEAGAELGEVYRTLWQYGLTLPAGTIANVGLTGLTLGGGIGLLTRAAGLTCDSLVQLEMIVADEKEGADLITVSCSNHPDLFWASQGGGGGNFGIVTSMTFKAVPISQVSIFSITWGWDDFEEVYNTWQN----------WAPYTDDRLTSSIEFWPKEVNR---IEALGQFVGPKTELKKLLKPLLKAGSPTSGMVKTTPFIEAVTFFNSPGGNQPQKMKRSGSFIEKPLSERAISTIKHFLEHAPNQNASVWQQA---LGGAAGRVAPDQTAFYYRDAIIAQEYLTNWTSPGEKRQNVRWI-EGLRTSLSKETMGDYVNWPDIEIRNWPRTYYG-ENVERLRRVKTTYDPENVFRFEQSIPPLRRSLFF 4XLO Chain:C ((20-461)) -------GELIWPSDADYDEARRIWNGTIDRRPALIARCTSTPDVVAAVSFARKSGLLVAVRGGGHSMAGHSVCDGGIVIDLSLMNSIKVSRRLRRARAQGGCLLGAFDTATQAHMLATPAGVVSHTGLGGLVLGGGFGWLSRKYGLSIDNLTSVEIVTAD----GGVLTASDTENPDLFWAVR-GGGGNFGVVTAFEFDLHRVGPVRFASTYYSLDEGPQVIRAWRDHMATAPDELTWALYL--RLAPPLPELPADMHGKPVICAMSCWIGDPHEGERQLESILHAGKP-HGLTKATLPYRALQAYSFPGAVVPDRIYTKSGYLNE-LSDEATDTV---LEHAADIASPFTQLELLYLGGAVARVPDDATAYPNRQSPFVTNLAAAWMDPTEDARHTAWAREGYR-ALAGHLSGGYVNFMNPGEADRTREAYGAAKFERLQGVKAKYDPTNLFRLNQNIPP-------

General information: TITO was launched using: RESULT: Template: 4XLO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2563 -199206 -77.72 -469.83 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -77.72 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_4XLO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XLO-query.scw PDB file : Tito_Scwrl_4XLO.pdb: