Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIVALHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLEELFEAYKKGKFFFITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQNNMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLK 4PZK Chain:B ((2-156)) ----GVHVVLYQPEIPANTGNIARTCAATGTELHLIRPLGFSTDDKMLKRAGLDYWQHVKITYYDSIEEFYEKNKDGEFFYLTKYGEKAHTAFDYSKREKDYYFVFGRETNGLPANVIEENFDHCLRIPMTDKVRSLNLSNTAAILIYEAFRQQNYPGL-

General information: TITO was launched using: RESULT: Template: 4PZK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 722 -116281 -161.05 -750.20 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.93 3D Compatibility (PKB) : -161.05 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.643

(partial model without unconserved sides chains): PDB file : Tito_4PZK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PZK-query.scw PDB file : Tito_Scwrl_4PZK.pdb: