Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIWTEDASR------MEAM-SLDYFLSYPRLFWPKFKELFQMKMSGSFEPNEGHLLLAELEKQGKQVDIFTQNIDGLHKKAGSRHVYELHGSIQTAACPACGARYDLPHLLER----EVPECTAAGNNGDICGTVLKTDVVLFGDAV-MHFDTLYEKL-DQADLLLVIGTSLEVAPARFVPEDASLIPGMKKVIINLEPTY-----------CDSLFDMVIHQKIGEFARSLGMKK------------------------- 5BTR Chain:A ((4-390)) DNLLFGDEIITNGSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQESKKIIVLTGAGVSVSSGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIY----PGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLA--IMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSS--IPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLSSNPVKLSEITEQYLFLPPNRYIFHGAEVY

General information: TITO was launched using: RESULT: Template: 5BTR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1190 -135887 -114.19 -568.56 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -114.19 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_5BTR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BTR-query.scw PDB file : Tito_Scwrl_5BTR.pdb: