Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLIEHYVALVKTGCAEYGQMNEMTLTEIADCLFCTERNAKLILHKLENSNWIIRESGAGRGRKSKIAFLRRPEELLLQTAKEYTMSGKLKKAKELLQQYQSAFPGLQNEYNMWLSEVFGFVTETGENGAKDVLRLFITPESVSSLDPCQIFLRSEGHFVKQIFDTLFTFDSDM----QEPKPHLVHG-WEEVG-KKQWRFFLRKGVLFHNGQPLTSRDVAFTFQRFLELA-DN-PYKW-----------------LLHGVKQVLEKGPYCVELILDKPNALLPYALCDERLSILPAEQG------GGKNGTGPFQMNQQHS-GMLVLEANERYFKG-----RPYLDRVEFVFSEQAGE-M-N--GFTIQ-E------K---Q--TCPEQQT--VFDERH---VQYLSLNLKKKGPLQHRSFRKALRLLISSERLVREAGGH--RRIPVTSFLHPSP--------------FEWEGVSPSELLKKSGYEG-ETIVLYTFSETDHREDAEWIQNICAQHGIRLTLQFCDAADLRRPE-------IVQMADIIHDSATFYQDSEFGFLHLLLSEN-------SFLYQHLSEKLTQICSGMTERMFSMPDRCSRINILRDIDRQMIQELNAIPLYQNVLQVTSSKNVKGLVLDE-EGWIDLYSVWLSK 2WOL Chain:A ((15-560)) ---------------------------------------------------------------------------------------------------------------AGWDAGVGALVNPS--RRRGGTLRLVS-SADVDSLDPARTYYVWVWLLQRLLNRTLMAYPTDPGPAGLVPAPDLAEGPGEVSDGGRTWTYRLRRGLRYDDGTPITSDDVRHAVQRVFAQDVLPGGPTYLIPLLDDPERPYPGPYRTDEPLRSVLTPDEHTIVFRLTRPFSDFDHLMAQPCAAPVPRRSDTGADYGRDPRSSGPYRVARHEPDTLLHLERNPHWDRATDPIRPALPDRVELTIGLDVDVLDARLIAGEFDINLEGRGLQHAAQRRATADEVLRSHTDNPRTSFLHFVAMQPHI-PPFDNVHVRRAVQYAADKILLQDARGGPVNGGDLTTALFPPTLPAHQDLDLYPTGPDLRGDLDAARAELAAAGLPDGFRAVIGTQ-RGKFRLVADAVVESLARVGIELTVKELDVATYFSLGAGHPETVREHGLGLLVTDWGADFPTEYGFLAPLVDGRQIKRNGGNWNLPEL---DDPEVNALIDETLHTTDPAARAELWRAVERRVMEHAVLLPLVHDKTLHFRNPWVTNVYVHPAFGLYDIQAMGLAE

General information: TITO was launched using: RESULT: Template: 2WOL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2490 -188127 -75.55 -412.56 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -75.55 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_2WOL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WOL-query.scw PDB file : Tito_Scwrl_2WOL.pdb: