Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGNEPHGGVLINRCDPACHF--------EGCACQAELDQLALSDLELIAIGGYSPLTGFLGEKDYHSVVKEMRLANGLPWSLPITLPVGEKTA--RQLSAGDHVKLVK--DGVTYGMITVTDIYQPDKTQEALSVFKTNDPAHPGVKKLLA-RPDYYIGGPITVSSLPD-KSFEQFYATPAETRAAFQKLGWKTIVGFQTRNPVHRAHEYIQKTALET-VDGLLLHPLVGETKSDDIPSDIRMESYQALLNHYYPKDRVMLSVFPAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVG------SYYGTYDAQNIFQSFTEEELGIKPLFFEHSFYCRKCGNMGTSKTCPHSPRDHIHLSGTKVRELLRQGKKPPKEFSRPEVAAVLIKGLHQQPVAIKQNSGELQ 1M8P Chain:A ((2-391)) --ANAPHGGVLKDLLARDAPRQAELAAEAESL-PAVTLTERQLCDLELIMNGGFSPLEGFMNQADYDRVCEDNRLADGNVFSMPITLDASQEVIDEKKLQAASRITLRDFRDDRNLAILTIDDIYRPDKTKEAKLVFGG-DPEHPAIVYLNNTVKEFYIGGKIEAVNKLNHYDYVALRYTPAELRVHFDKLGWSRVVAFQTRNPMHRAHRELTVRAARSRQANVLIHPVVGLTKPGDIDHFTRVRAYQALLPRY-PNGMAVLGLLGLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGSNSKGEDFYGPYDAQHAVEKYK-DELGIEVVEFQMVTYLPDTDEYRPVDQVPAG-VKTLNISGTELRRRLRSGAHIPEWFSYPEVVKILRESNPP-------------

General information: TITO was launched using: RESULT: Template: 1M8P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2024 -190880 -94.31 -517.29 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -94.31 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_1M8P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M8P-query.scw PDB file : Tito_Scwrl_1M8P.pdb: