Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKLLAFALCTGAYAALFAYSVNSEQKTATSEMTDVSRLMPVKIKQTVKGQEEEMLIDTVKEANRKNIKISIA-GAQHSMGGHTYYEDGIVLDMTGYNKILSLDQEKKTIRVQSGATWNDIQKYVNPYGLAVKVMQSQNIFTIGGSLSANAHGRDIR-YGSLIDTVKSFRLLKADGMIITVTPK---DDLFTAVIGGYGLFGVILDVTLELTD----------------DELYVMKTE--KMNYSTYSDYFSKHVKGNPDVRMHLARISTAKKGFLKDMYVTNYVLANHQDQLSSYSELKEDE---YTGATKFALGLSRRYEWGRNWLWDTQQSYFLSQNGTEISRNNVMRSESKFLEYENNDNTDVLQEYFVP---VKEYGSYIDDLRQTLSDEDLNLLNITIRYVQKNEKADLSYAKDDMFSLV-LLINEGFSKEDQADTARIIRRMTDVAIKHGGSYYLPYMTYQTKAQMRQAYPKSEAFFQKKRTYDPDERFMNYFYQRYK 4F4Q Chain:A ((15-466)) ---------------------------PTTTKRLMGWGRTAPT-VASVLSTSDPEVIVRAVTRAAEEGGRGVIARGLGRSYGDNAQNGGGLVIDMPALNRIHSIDSGTRLVDVDAGVSLDQLMKAALPHGLWVPVLPGTRQVTVGGAIGCDIHGKNHHSAGSFGNHVRSMELLTANGEVRHLTPAGPDSDLFWATVGGNGLTGIILRATIEMTPTETAYFIADGDVTGSLDETIAFHSDGSEANYTYSSAWFDAISKPPKLGRAAISRGSLAK-----------------LDQLP--SKLQKDPLKF--------------------WYRKSGTYRNKVQNLTQFYHP-------GFLQY----------QFVVPTEAVEEFKSIIVDIQRSGHYSFLNVFKL----FGPGNQAPLSFPIPGWNVCVDFPIKAGLHE--------FVTELDRRVLEFGGRLYTAKDSRTTAETFHAMYPRIDEWIRIRRSVDPDGVFASDMARRLQ

General information: TITO was launched using: RESULT: Template: 4F4Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2135 -174485 -81.73 -459.17 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -81.73 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_4F4Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F4Q-query.scw PDB file : Tito_Scwrl_4F4Q.pdb: