Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------MSDNDKFKEELAKL--------PEVDPMTKMLVQNIFSKHGVTKDKMKK--VSDEEKEMLLNLVKDLQAKSQALIENQKKKKEEAAAQEQKNTKP-------------LSRREQLIEQIRQRRKNDNN- 2P7V Chain:A ((1-158)) MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQIMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA

General information: TITO was launched using: RESULT: Template: 2P7V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 233 -11666 -50.07 -111.10 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -50.07 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.660

(partial model without unconserved sides chains): PDB file : Tito_2P7V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P7V-query.scw PDB file : Tito_Scwrl_2P7V.pdb: