Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSHSQEKIALITGASSQGDIGTAICRKLASQGIHIFFTHWNSDTAWIEEFQQEILRMGVRCEAMKIDLSDAHAAFTIHEKISDKLGYPSILINNAAHSASDNYVSLDAKSLDEHYAVNMRSNFLLCVEFARRFKKSNLISGRIINMTSGQDLGPLPGELAYAATKGAISAFTRSLSQELAPLGITVNAVNPGPTDSTWMTDEI----RNFLSPKFPMGRIGTPDDAARMIAFLASDEAEWITGQIIHSEGGFIRG 4BNZ Chain:B ((26-266)) ----QGKVALVTGASR--GIGQAIALELGRLGAVVIGTATSASGA--EKIAETLKANGVEGAGLVLDVSSDESVAATLEHIQQHLGQPLIVVNNAGITRD--------DEWFDVVNTNLNSLYRLSKAVLRGMTKARW--GRIINI--GSVV---AGQTNYAAAKAGLEGFTRALAREVGSRAITVNAVAPGFID-TDMTRELPEAQREALLGQIPLGRLGQAEEIAKVVGFLASDGAAYVTGATVPVNGGM---

General information: TITO was launched using: RESULT: Template: 4BNZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1205 -145333 -120.61 -648.81 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -120.61 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_4BNZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BNZ-query.scw PDB file : Tito_Scwrl_4BNZ.pdb: