Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGGRLKSLRG--KRTQEEIASHIGVSRAR----YSHYENGRSEPDYDTLQKLADYFQVTTDYLLTGKDKKSDDDMFSDPDLQLAYRDMQDFSPESKQQAIEFINYLKEKEKNRKPKNK 4O8B Chain:A ((1-101)) MLGTRLKAARIRAGYSQKQLGMLVGMDEFSASARMNQYERERHSPNMRTSEQLAMVLQVPMAYLYCPEDEL-----------AELILKVSSLTPEFKKELTRFIEQLLAAQG-------

General information: TITO was launched using: RESULT: Template: 4O8B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 271 -29042 -107.17 -305.71 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -107.17 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_4O8B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O8B-query.scw PDB file : Tito_Scwrl_4O8B.pdb: