Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTILLSLAILVFVPFLYKANRNTKDVKVNTISIDQKDGLPARKKLNILHLSDLHLENIS--------------VSPEELYHLTKDQPVDIIALTGDFLDRKRN-I-PKLAGYLNALQKLKPAYGMYAVFGNHDYVLKEEDFQRLKRVLEENGCITL-QNEHVRIET-AAGPVNIIGIDDYSTNRS--------------NITGSYQS-L-ENGYHLVLTHDPNIIL----------------DMKDVHYDYLLSGHFHGGQIHWPKPYHLVKMGKLVRMNMIKGLHYHHDKPFYISEGLGQTGVNIRVGSRPEVTFHQI 4NZV Chain:A ((16-236)) ------------------------------------------LKELKILHTSDWHLGVTSWTSSRPVDRREELKKALDKVVEEAEKREVDLILLTGDLLHSRNNPSVVALHDLLDYLKRMMRTAPVVVLPGNHDWKGLKL-FGNFVTSI-SSDITFVMSFEPVDVEAKRGQKVRILPFPYPDESEALRKNEGDFRFFLESRLNKLYEEALKKEDFAIFMGHFTVEGLAGYAGIEQGREIIINRALIPSVVDYAALGHIHSFREIQ-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4NZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 796 -104108 -130.79 -608.82 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -130.79 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_4NZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NZV-query.scw PDB file : Tito_Scwrl_4NZV.pdb: