Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTKRLLTIYIMLLGLIAWFPGAAQAEEKQPAVPAVFLMKTIEGEDISIPN-KGQKTILHFWTSWCPPCKKELPQFQSFYDAHPSDSVKLVTVNLVNSEQNQQVVEDFIKANKLTFPIVLDSKGELMKEYHIITIPTSFLLNEKGEIEKTKIGPMTAEQLKEWTEE 2H19 Chain:A ((9-132)) ------------------------------------FVLEDTNGKRIELSDLKGKGVFLNFWGTWCEPAKKEFPYMANQYKHFKSQGVEIVAVNV---GESKIAVHNFMKSYGVNFPVVLDTDRQVLDAYDVSPLPTTFLINPEGKVVKVVTGTMTESMIHDY---

General information: TITO was launched using: RESULT: Template: 2H19.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 548 -79236 -144.59 -644.19 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -144.59 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.671

(partial model without unconserved sides chains): PDB file : Tito_2H19.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H19-query.scw PDB file : Tito_Scwrl_2H19.pdb: