Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLMGIITRLQSLQETAEAA-NEPMQRYFEVNGEKICSVKYFEKNQTFELTVFQKGEKPNTYPFDNIDMVSIEIFELLQ 2FFG Chain:A ((2-79)) -SQLMGIITRLQSLQETA-EAANEPMQRYFEVNGEKICSVKYFEKNQTFELTVFQKGEKPNTYPFDNIDMVSIEIFELLQ

General information: TITO was launched using: RESULT: Template: 2FFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 268 -48206 -179.87 -626.05 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.98 3D Compatibility (PKB) : -179.87 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.731

(partial model without unconserved sides chains): PDB file : Tito_2FFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FFG-query.scw PDB file : Tito_Scwrl_2FFG.pdb: