Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKALKALGVLTTFVMLIVLI-GGALVTKTGSGQGCGRQWPLCH-GRFFPELNPASIIEWSHRFASGISIILVLSLAFWSWRKITPIFRETTFLAIMSIIFLFLQALLGALAVVFGSN-ALIMALHFGISLISFASVLILTLLIFEADKSVRTLVKPLQIGKKMQFHMIGILIYSYIVVYTGAYVRHTESSLACPNVPLCSPLNNGLPTQFHEWVQMGHRAAALLLFVWIIVAAVHA-ITSYKDQKQIFWGWISC-LI-FITLQALSGIMIVYSELA-LGFALAHSFFIACLFGVLCYFLLLIARFRYESR-QS 2EYU Chain:A ((6-251)) --PEFKKLGLPDKVLELCHRKMGLILVT---GPTGSGKSTTIASMIDYINQTKSYHIITIED------------PIEYVFKHKKSIVNQREVGEDTKSFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGTLHTNTAIDTIHRIVDIFP--LNQQEQVRIVLSFILQGIISQRLL----------------PKIGGGRVLAYGLL-IPNTAIRNLIRENKLQQVYSLMQSGQAETG---------MQTMNQTLYKLYKQGLITLEDAMEASP----------------------DPKELERMIR

General information: TITO was launched using: RESULT: Template: 2EYU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1245 -185864 -149.29 -780.94 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -149.29 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_2EYU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EYU-query.scw PDB file : Tito_Scwrl_2EYU.pdb: