Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIKDLMTA--DLQYCTVLDNVYEAAVKMKDANVGAIPVVDEDGETLVGIVTDRDLVLRGIAIKK----------PNSQKITDAMTEKPVSVEEDASVDEVLHLMASHQLRRIPVTK-NKKLTGIVTLGDLSLSEQTNERAGSALSDISEGDNREEGFFH 4GQW Chain:A ((75-227)) -YTVGEFMTKKEDLHVVKPTTTVDEALELLVENRITGFPVIDE-DWKLVGLVSDYDLLALD----TWKTFNAVQKL--GKLVGDLMTPAPLVVEEKTNLEDAAKILLETKYRRLPVVDSDGKLVGIITRGNVVRAALQ-----------------------

General information: TITO was launched using: RESULT: Template: 4GQW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 547 -73131 -133.69 -625.05 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -133.69 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_4GQW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GQW-query.scw PDB file : Tito_Scwrl_4GQW.pdb: