Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQHKTVLLRETVDGLNIKPDGTYVDCTLGGAGHSTYLLQQLSEKGRLIAFDQDDTALQHAKEVLSDYKGQLILIKSNFRYLKEYLNEQGVTEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSATLSAKEVVNEWRYEDLVRIFFKYGEEKFSKQIARKIEEARMKSPIQTTGQLVDLIKDAIPAPARRSGGHPAKRVFQAIRIAVNDELRVFEEALEQAIEVLKPGGRVSVITFHSLEDRICKTTFKEKSSLPELPPGLPVIPEEFEPELKLITRKPITASQEELEENNRARSAKLRIAEKRK 1WG8 Chain:A ((4-282)) -MTHVPVLYQEALDLLAVRPGGVYVDATLGGAGHARGILER---GGRVIGLDQDPEAVARAKGL---HLPGLTVVQGNFRHLKRHLAALGVERVDGILADLGVSSFHLDDPSRGFSYQKEGPLDMRMGLEGP-TAKEVVNRLPLEALARLLRELGEEPQAYRIARAIVAAREKAPIETTTQLAEIVRKAV---GFRRAGHPARKTFQALRIYVNDELNALKEFLEQAAEVLAPGGRLVVIAFHSLEDRVVKRFLRESG-------------------LKVLTKKPLVPSEKEAAQNPRARSAKLRAAEK--

General information: TITO was launched using: RESULT: Template: 1WG8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1553 -139211 -89.64 -498.96 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -89.64 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_1WG8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WG8-query.scw PDB file : Tito_Scwrl_1WG8.pdb: