Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKKAYLTVCSNQQIADRVFQMVLKGELVQGFTTPGQFLHLKVSEAVTPLLRRPISIADVNFEKNEVTIIYRVDGE--GTRLLSLKQQGELVDVLGPLGNGFPVNEVQPGKTALLVGGGVGVPPLQELSKRLIEKGVNVIHVLGFQSAKDVFYEEECRQYG--DTYVATADGSYGETGFVTDVIKRKKLEFDILLSCGPTPMLKALKQEYAHKE-VYLSMEERMGCGIGACFACVCHTNESETSYVKVCLDGPVFKAQEVAL 1EP3 Chain:B ((2-262)) SQLQEMMTVVSQREVAYNIFEMVLKGTLVDEMDLPGQFLHLAVPNG-AMLLRRPISISSWDKRAKTCTILYRIGDETTGTYKLSKLESGAKVDVMGPLGNGFPVAEVTSTDKILIIGGGIGVPPLYELAKQLEKTGCQMTILLGFASENVKILENEFSNLKNVTLKIATDDGSYGTKGHVGMLMNEIDFEVDALYTCGAPAMLKAVAKKYDQLERLYISMESRMACGIGACYACVEHDKEDESHALKVCEDGPVFLGKQLSL

General information: TITO was launched using: RESULT: Template: 1EP3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1496 -164863 -110.20 -646.52 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -110.20 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_1EP3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EP3-query.scw PDB file : Tito_Scwrl_1EP3.pdb: