Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLAPHGGTLVN-------RVDESYDVSGIQKEIELDLISFADLELIGIGAYSPIEGFFNEKDYVSVVENMRLSSGVVWSLPITLPVDAQKAAELSLGETVKLTY----EGETYGVIQIEDLYVPDKQKEAVNVYKTDEQEHPGVKKLFSRGNT----YVGGPITLIKKASK-QFPEFTFEPSETRRQFAEKGWETIVGFQTRNPVHRAH-EYIQKTALETVDGLFLNPLVGETKSDDIPADVRMESYQVLLDHYYPKDRVFLGVFLAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVG------DYYGTYEAQELFDTFKPEELGITPLKFEHSFFCKKCGNMGTAKTCPHGREHHVILSGTKVRGMLRDGVLPPAEFSRKEVVEVLIKGMKKKEEVGVS 1I2D Chain:C ((2-385)) -ANAPHGGVLKDLLARDAPRQAELAAEAESLPAVTLTERQLCDLELIMNGGFSPLEGFMNQADYDRVCEDNRLADGNVFSMPITLDASQEVIDEKKLQAGSRITLRDFRDDRNLAILTIDDIYRPDKTKEAKLVFGGDP-EHPAIVYL---NNTVKEFYIGGKIEAVNKLNHYDYVALRYTPAELRVHFDKLGWSRVVAFQTRNPMHRAHRELTVRAARSRQANVLIHPVVGLTKPGDIDHFTRVRAYQALLPRY-PNGMAVLGLLGLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGSNSKGEDFYGPYDAQHAVEKYK-DELGIEVVEFQMVTYLPDTDEYRPVDQVPAGVK-TLNISGTELRRRLRSGAHIPEWFSYPEVVKIL-------------

General information: TITO was launched using: RESULT: Template: 1I2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1974 -198779 -100.70 -550.63 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -100.70 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_1I2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I2D-query.scw PDB file : Tito_Scwrl_1I2D.pdb: