Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFPKKAEIIAVGSELLLGQIANTNAQFISKQLAEIGVNVFYHTAVGDNPERLKQVIRIAEERSDFIIFSGGLGPTKDDLTKETIANTLGRPLVLNDEAFQSIEDYFKRTKRTMSPNNRKQALVIEGSDVLANHFGMAPGMLTEHESRYYMLLPGPPSELRPMFENEAKPLLLKKMGSNEKIVSTVLRFFGIGESQLEADLEDI------IDAQTNPTIAPLAADGEVTLR----LTAKHADEKETERLLKETEAVILERVGEFFYGYDDTSLVKELSIACKEKGITISAAESFTGGLFSEWLTDHSGASKLFAGGVVCYTNDVKQNVLGVKKETLDRFGAVSKECASELAKGVQKLTGSDIGISFTGVAGPDAQEGHEPGHVFIGISANGKEEVHEFHFAGSRTGIRKRGAKYGCHLILKLLEQK 4UUX Chain:A ((2-393)) ----ERAEILGVGTELLYGETLDTNTAEIARSLKPYALKVERTLRVADEVAPLAREVEEAFARARLVVLSGGLGPTPDDVTREAVALALGEPLELDEAVLGEIEAFFRARGRAMPEANRKQAMRIPSATWLKNPRGTAPGWWVRKGGKDLVLLPGPPPEWRPMWQEVLPRLGLPRRPYAERVLKT----WGIGESEIVERLGPLFVRGEEVEVGTYPKVHGV----EVVVRGREDRVAELA-ERIKKKLLKE------------VWGEGEMTLAEAVKRRMEREGATLSTMESLTGGLLGAEITRVPGASRFYLGGVVSYSVGAKAR-FGVPQDLLSR--TVSAETARAMAEAARSLFGSTYALATTGVAGPDPLEGEPPGTVYVALAGPTGAEVRRYRFPGDRETVRLRSVYAALALLV------

General information: TITO was launched using: RESULT: Template: 4UUX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2050 -177316 -86.50 -464.18 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -86.50 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_4UUX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UUX-query.scw PDB file : Tito_Scwrl_4UUX.pdb: