Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSPTRRRTAKRRRRKLNKRGKLLFGLLAVMVCITIWNALHRNSEENEPSQETAAVSNTDQKKEVKKKTAKKSEEQIKTVDRNQKISNYLKEIGFSGTAMIVRNGEIVTNKGFGYADRKHYIQNNPLTSFYVGSSQKALIATAILQLEEKGKLQTSDPVSTYLPH-FPNGQTITLKNLLTHTSGINGHIEGNGAITPDDLIKDIELQGIKR---------------QPG-VWDYKDSNYSVLAYIIAEVSGEPYEQYIKNHIFKPAGMTHAGFYKTYEKEPYPAVGY--KMEGSKTVTPYIPDLSQLYGA-GDIYMSAIDMYKFDQALIDGKLYSQKSYEKMFTPG----SSSTYGMGFYV--APGSYSNHGVM---PGFNILNSFSKSGQTIVILFSNIQNNAKLGQVNNKIYQLLNQE 3TG9 Chain:A ((9-318)) -----------------------------------------------------------------------------------------MEDWKLSGTALMKKGEDIPFIASLGFANRAERIPNEHHTRFGIASGCKLFTAIAICQLVEAGKLSFDTPLSDWLDAPFPN---VTIHHLLTHTSGVPDYFD-------EDLWKDVPMYHLRRLKDFLPLFQHAPMKFPPGHRFHYNNAGFILLGLVVESVSGVTFQEYVEANVFQRAGMHESGYFAFDTLPAKTALGYIDLEDGSWKTNLY--SLPVIGGSDGGAYVTAEDMMKLWLALMRHELLNETYTQKLLTPHVHCEDDDYYGYGVWIKQQDGAISKYHVMGYDPGVCFHSAFYPTSNGIVVVCANQSSGA----------------

General information: TITO was launched using: RESULT: Template: 3TG9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1534 -177896 -115.97 -649.25 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -115.97 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_3TG9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TG9-query.scw PDB file : Tito_Scwrl_3TG9.pdb: