Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAQNNENALPDITKSITLEAPIQKVWETVSTSEGIAKWFMPNDFQLKEGQEFHLQSPFGPSPCKVLAVQAPTELSFEWDTEGWVVTFQLEDLGEKTGFTLIHSGWKEPNEVIGKANEKSSVVRGKMDGGWTGIVNERLRKAVEE 2KEW Chain:A ((1-144)) MAQNNENALPDITKSITLEAPIQKVWETVSTSEGIAKWFMPNDFQLKEGQEFHLQSPFGPSPCKVLAVQAPTELSFEWDTEGWVVTFQLEDLGEKTGFTLIHSGWKEPNQVIGKANEKSSVVRGKMDGGWTGIVNERLRKAVEE

General information: TITO was launched using: RESULT: Template: 2KEW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 770 -48071 -62.43 -333.82 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -62.43 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_2KEW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KEW-query.scw PDB file : Tito_Scwrl_2KEW.pdb: