Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNPSKAEIKEILQRSKRIAVVGLSDRPDRTSHMVSKAMQDAGYEIIPVNPTIDEALGVKAVSSLKEIDGPIDIVNVFRRSEQLPGVAEEFLETDAPVFWAQQGLVNEEAYQMLKEKGRTVIMDLCIKVAHAVTK 2D59 Chain:A ((12-140)) -----DEDIREILTRYKKIALVGASPKPERDANIVMKYLLEHGYDVYPVNPKYEEVLGRKCYPSVLDIPDKIEVVDLFVKPKLTMEYVEQAIKKGAKVVWFQYNTYNREASKKADEAGLIIVANRCMMREHERL-

General information: TITO was launched using: RESULT: Template: 2D59.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 690 -92018 -133.36 -713.31 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -133.36 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.725

(partial model without unconserved sides chains): PDB file : Tito_2D59.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2D59-query.scw PDB file : Tito_Scwrl_2D59.pdb: