Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSFATQQNGIFKSVCSLDCPDQCGLLIHKKDGKIVKVQGDPDHPVTAGNICNKVRNMTERIYDEKRLTTPLKRTGAKGQAIFEPISWKEAIDTITSRWKQLIDEEGAESILPYSFYGNMGKLTAEG--MDRRFFYRMGSSQLERT--ICSKAGSEGYKYT------MGISAGIDPEETVHTKLFIFWGINAVSTNMHQITIAQKARKK----GAKIVVIDVHKNQTGRLADWFIPIKPGTDSALALGIMHILFKENLHDEAFLSEYT---------VGYEELREHVKQYDPEKVSTITGVSTEDIYRLAKMYGETSPSFIRIGNGPQHHDNGGMIVRTIACLPAITGQWLHTG------------------GGAIKHNSGILEYNTNALQRPDLLK------GRTP--RSFNMNQLGRVLLETDPPIRSLFIYGTNPAVVAPEANKVRQGLLREDLFTVVHDLFLTE-TAAYADIVLPATSAFENTDFYTSYWHHYIQLQQPVIERYGESKSNTEVFRLLAEAMGFTDQ--ELKDSDEVLIRQALDHPDNPHLAEIDYDSLTKHSFMK--------------------AKREKPLFPGELP-------TPSGKIELYSEKMKQDGFPALPTYTPLVTDNEHPFMYVPGPNHNFLNSTFSNNEKHIKLEKT-PKLF--INTKDAEKHGIVDGAPVRIWNSRGECELTAAVGEQVLPGVVVSQGLWADEQGKKQLVNALTPDRLSDMGGGATFFSGRVQIEKV 2V3V Chain:A ((10-702)) ------------KGVCRY-CGTGCGVLVGVKDGKAVAIQGDPNNH-NAGLLCLKGSLLIPVLNSKERVTQPLVRRHKGGK--LEPVSWDEALDLMASRFRSSIDMYGPNSV---AWYGSGQCLTEESYVANKIFKGGFGTNNVDGNPRLCMASAVGGYVTSFGKDEPMGTYADID-------QATCFFIIGSNTSEAHPVLFRRIARRKQVEPGVKIIVADPRRTNTSRIADMHVAFRPGTDLAFMHSMAWVIINEELDNPRFWQRYVNFMDAEGKPSDFEGYKAFLENYRPEKVAEICRVPVEQIYGAARAFAESAATMSLWCMGINQRVQGVFANNLIHNLHLITGQICRPGATSFSLTGQPNACGGVRDGGALSHLLPAGRAIPNAKHRAEMEKLWGLPEGRIAPEPGYHTVALFEALGRGD--VKCMIICETNPAHTLPNLNKVHKAMSHPESFIVCIEAFPDAVTLEYADLVLPPAFWCERDGVYGCGERRY-SLTEKAVDPPGQCRPTVNTLVEFARRAGVDPQLVNFRNAEDVWNEWRMVSKGTTY----DFWGMTRERLRKESGLIWPCPSEDHPGTSLRYVRGQDPCVPADHPDRFFFYGKPDGRAVIWMRPAK--GAAEEP-------DAEYP-LYLTSMRVIDHWHTATMTGKVPELQKANPIAFVEINEEDAARTGIKHGDSVIVETRRDAMELPARVSDVCRPGLIAVPFF-----DPKKLVNKLFLD---------------------

General information: TITO was launched using: RESULT: Template: 2V3V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3895 28133 7.22 46.04 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 7.22 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_2V3V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V3V-query.scw PDB file : Tito_Scwrl_2V3V.pdb: