Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLNQKALNSYVYTLAFSSLSFGLI---FGLYLFV-----YSGFMA---------IALVTIAIIAFYALITYLVFAAPL--QVWLRRRRRKFSLIN-FLIYIAVAFSAVFLFWFVDYPPNALTMFRSFEYYIMSIVAAFIYWFWDSIFLRN 2Y00 Chain:A ((43-177)) ----QTLTNLFITSLACADLVVGLLVVPFGATLVVRGTWLWGSFLCELWTSLDVLCVTASIETLCVIAIDRYLAITSPFRYQSLMTRARAKVIICTVWAISALVSFLPIMMHWWRDEDPQALKCYQD------------------------

General information: TITO was launched using: RESULT: Template: 2Y00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 260 -51266 -197.18 -497.73 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -197.18 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_2Y00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y00-query.scw PDB file : Tito_Scwrl_2Y00.pdb: