Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------MSTIKVK-SAHKDGQIKLEDLDVVCNKLCKRNNSVLFKLEKYLNK--KLLSDPELTEIRDTILTVSGEL-----SRLRDNLVT-DGDSNEGLQ------------ 4G3A Chain:A ((5-231)) KPSDLDGFIQQMPKADMRVKVQLAEDLVTFLSDDTNSIVCTDMGFLIDGLMPWLTGSHFKIAQKSLEAFSELIKRLGSDFNAYTATVLPHVIDRLGDSRDTVREKAQLLLRDLMEHRVLPPQALIDKLATSCFKHKNAKVREEFLQTIVNALHEYGTQQL-SVRVYIPPVCALLGDPT-VNVREAAIQTLVEIYKHVGDRLRPDLRRMDDVPASKLAMLEQKFDQVKLE

General information: TITO was launched using: RESULT: Template: 4G3A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 272 -628 -2.31 -7.65 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -2.31 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_4G3A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G3A-query.scw PDB file : Tito_Scwrl_4G3A.pdb: