Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFHDDKKNELQKKEEIITEAIDTLFQSSAFGNLINGFQNLINSSLKDVQTTIHVRERDTGLYIDITIPATFRDGEIVVDVKSRYLHVTLQEKQKHQNEATFTSMTRTVQLPYEVRQEDMETSWN-EQTMTLFFPKNKHE 4YDZ Chain:A ((30-126)) --------------------------------SMLNNFNNIVPQQLNEVENT----AQKFCVKLDV---AAFKPEELKVNLEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQMTIDAPK----

General information: TITO was launched using: RESULT: Template: 4YDZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 361 -31786 -88.05 -331.10 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -88.05 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_4YDZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YDZ-query.scw PDB file : Tito_Scwrl_4YDZ.pdb: