Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNKIYDWVDERLDITPMWRDIADHEVPEHVNPAHHFSAFVYCFGGLTFFVTVIQVLSGMFLTMYYVPDIKNAWESVYYLQNEVAFGQIVRGMHHWGASLVIVMMFLHTLRVFFQGAYKKPRELNWIVGVLIFFVMLGLGFTGYLLPWDMKALFATKVGLQIAEATPLIGTQVKTLLAGHPDIVGAQTLTRFFAIHVFFLPAALFGLMAAHFIMIRKQGISGPL 4H0L Chain:A ((3-215)) ---NVYDWFQERLEIQALADDVTSKYVPPHVN-------IFYCLGGITLTCFLIQFATGFAMTFYYKPTVTEAYASVQYIMNEVSFGWLIRSIHRWSASMMVLMMILHVFRVYLTGGFKKPRELTWISGVILAVITVSFGVTGYSLPWDQVGYWAVKIVSGVPEAIPVVGVLISDLLRGGSS-VGQATLTRYYSAHTFVLPWLIAVFMLLHFLMIRKQGISGPL

General information: TITO was launched using: RESULT: Template: 4H0L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 707 -121829 -172.32 -571.97 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -172.32 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_4H0L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H0L-query.scw PDB file : Tito_Scwrl_4H0L.pdb: